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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.
Brand: Thermo Scientific™ A39258
This item is not returnable.
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Description
Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.
- Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection
- Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Cleavable - disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
- Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Medium length - spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
Specifications
| Sulfo-NHS-SS-Biotin | |
| Store at -20°C. | |
| Wet Ice | |
| Cell-Impermeant | |
| Sulfo-NHS Ester | |
| Biotin |
| 10 x 1 mg | |
| Chemical Labeling | |
| EZ-Link™ | |
| Amine | |
| DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water | |
| Cleavable, 24.3 Å |
For Research Use Only. Not for use in diagnostic procedures.