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Invitrogen™ Endothelin-1 (ET-1) Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EIAET1
Includes: Pre-coated plate(s), standard, peroxidase conjugated detector antibody, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1. Principle of the method The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
Specifications
| P05305 | |
| 0.579 pg/mL | |
| Multiplex assay on the Luminex Platform | |
| Fluorescence | |
| Peptide hormone | |
| 96 assays | |
| ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
| 1906 | |
| 3.7% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Cell Signaling | |
| -20°C | |
| 1 hr. 20 min. |
| 0.781-100 pg/mL | |
| Endothelin-1 (ET-1) ELISA Kit | |
| HRP | |
| ELISA Kit | |
| Plasma, Serum, Supernatant, Urine | |
| Human | |
| Colorimetric Microplate Reader | |
| EDN1 | |
| 3.3% | |
| HRP | |
| RUO | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 2.5 μL; Urine,50 μL | |
| Human | |
| 2 hr. 30 min. |
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