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Diethyl Pyrocarbonate, MP Biomedicals™
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Quantity:
100 mL
Description
DEPC inactivates RNase in solution at about 0.1% (v/v). It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. DEPC is effective as a nuclease inhibitor with a variety of uses:
- Modification reagent for His and Tyr residues in proteins
- Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases
- Used to modify proteins and nucleic acids
- PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases
- Dot blot hybridization, to dilute total RNA isolated from different micro-organisms
Specifications
Specifications
CAS | 1609-47-8 |
Density | 1.101g/mL at 25°C (Lit.) |
Boiling Point | 93°C to 94°C (18 mmHg)(literature) |
Molecular Formula | C6H10O5 |
Refractive Index | n20/D 1.398 (Lit.) |
MDL Number | MFCD00009106 |
Quantity | 100 mL |
Synonym | diethyl pyrocarbonate, diethyl dicarbonate, depc, diethyl oxydiformate, baycovin, diethylpyrocarbonate, ethyl pyrocarbonate, dicarbonic acid, diethyl ester, dicarbonic acid diethyl ester, diethyl pyrocarbonic acid |
Solubility Information | Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2. |
InChI Key | FFYPMLJYZAEMQB-UHFFFAOYSA-N |
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Safety and Handling
Product Identifier
- Diethyl Pyrocarbonate
- Warning
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- P101-If medical advice is needed, have product container or label at hand.
- P102-Keep out of reach of children.
- P103-Read label before use.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : diethyl pyrocarbonate
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