The CellSensor™ NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells.
Brand: Thermo Scientific™ K1447
The CellSensor™ NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
| Division-Arrested Cells | |
| CellSensor™ NFAT CHO-K1 DA Assay Kit, 1Vial, ∽ 2,400,000 cells/vial Quantity of Cells, GeneBLAzer™ Druggable Target, Fluorescent Detection Method, Adherent Growth Properties, Liquid Nitrogen Storage | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, pathway biology, cellular pathway analysis assays, cellsensor™ cellular pathway assays | |
| CellSensor™ | |
| Division-Arrested Cells Assay Kit | |
| Signaling Pathway |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: • CellSensor™ NFAT CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • CellSensor™ NFAT CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The CellSensor™ NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescent | |
| beta-lactamase (bla) reporter gene under control of a nuclear factor of activated T cells (NFAT) response element stably integrated into CHO-K1 cells | |
| 1 Plate | |
| GeneBLAzer™ |
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A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 40644 | Certificate of Analysis | 15/04/2017 | K1447 |
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