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Molecular Probes™ CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells.
141.00€ - 473.00€
Specifications
Format | Kit |
---|---|
Product Type | Caspase Assay Kit |
Content And Storage | Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO. Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light. |
Flow Cytometer Laser Lines | 488 |
Shipping Condition | Wet Ice |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
13413329
|
Molecular Probes™
C10427 |
100 Assays |
473.00€
1 set |
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15583329
|
Molecular Probes™
C10740 |
20 Assays |
141.00€
1 set |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.
• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers
CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
Specifications
Kit | |
Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO. Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light. |
|
Wet Ice | |
Fluorescence | |
Attune™ Acoustic Focusing Cytometer, Flow Cytometer | |
Other Label(s) or Dye(s) |
Caspase Assay Kit | |
488 | |
CellEvent™ | |
Flow Cytometry | |
CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain |
For Research Use Only. Not for use in diagnostic procedures.
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