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![Thermo Scientific BacMam Akt [pSer473] Cellular Assay Kit 1 Kit:Cell Analysis](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-650.jpg)
Thermo Scientific™ BacMam Akt [pSer473] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Brand: Thermo Scientific™ A12899
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Description
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Ser473.
Spécification
| Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient) | |
| Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation. | |
| Fluorescence | |
| Kinases, Cell Signalling | |
| LanthaScreen™ | |
| End Point | |
| AKT1 | |
| LanthaScreen™ |
| Cells Not Included | |
| BacMam Akt [pSer473] Cellular Assay Kit, 1 Kit, Fluorescent Detection Method, Kinases Druggable Target, TR-FRET Technique, LanthaScreen™ System, End Point Readout, GFP (EmGFP) Reporter | |
| TR-FRET | |
| Terbium-labeled anti-AKT antibody, BacMam AKT virus and 6X lysis buffer | |
| 1 kit | |
| GFP (EmGFP) | |
| Cellular Assay Kit | |
| Kinases |
For Research Use Only. Not for use in diagnostic procedures.