Ig Goat anti-Rat, Brilliant Violet 421, Polyclonal, BD

Description

BD Horizon™ BV421 Goat Anti-Rat Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rat Ig primary antibodies. It also stains rat B cells and has little reactivity with rat non-B cells or mouse splenocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies although a weaker signal may be detected when the primary antibody has a rat IgM or IgG2b isotype due to its adsorption with mouse Ig. It has weak crossreactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. BD Horizon BV421 has an Ex Max at 407nm and Em Max at 421 nm. The use of a mounting reagent (eg, ProLong™ Gold) is highly recommended to maximize the photostability of BV421.

For epifluorescence microscopes with broad spectrum excitation sources, the recommended excitation and emission filters are 392/23nm and 430/24nm bandpass filters, respectively. For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging.

For flow cytometry, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter).