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Enzymes and Inhibitors

Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (361)
Modifying Enzymes (3)

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1 – 15 of 326 results
1

Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Promotions are available
Cut Site CTCTTC(1/4)^
Content And Storage Store at -20°C.
Form Liquid
Enzyme Eam1104I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity 3000 U, 150 μL
Type IIS RE Yes
Incubator Temperature 37°C
Concentration 20 U/μL
For Use With (Application) In vitro Transcription
Inactivation Temperature 65°C
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Research Category Drug Development
Compatible Buffer 10X Tango Buffer
Optimal Reaction Temperature 37°C
Product Line TheraPure™
2

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

Promotions are available
3

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

Promotions are available
4

Invitrogen™ RNase Cocktail™ Enzyme Mix

Endonuclease-free, exonuclease-free, protease-free

Promotions are available
Shipping Condition Dry Ice
Product Type RNAse Cocktail Enzyme Mix
Enzyme RNase
Product Line Ambion™
5

Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix

Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

Promotions are available
EDGE
Shipping Condition Dry Ice
Content And Storage Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type LR Clonase Enzyme Mix
Enzyme LR Clonase
Compatible Buffer Enzyme Buffer
Product Line Clonase™, Gateway™
6

Thermo Scientific™ FastDigest NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nde I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
7

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nco I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
8

Thermo Scientific™ FastDigest NheI

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nhe I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
9

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Promotions are available
EDGE
10

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

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11

Thermo Scientific™ FastDigest SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme SacI
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 3' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
12

Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

Promotions are available
EDGE
Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Bcu I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest™
13

Thermo Scientific™ HinfI, HC (50 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Hinf I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer R
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
14

Thermo Scientific™ FastDigest SgsI

Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).

Promotions are available
15

Thermo Scientific™ FastDigest Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).

Promotions are available
EDGE
Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Eco32I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang Blunt
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest™