Content And Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Target Species |
Human,Mouse,Rat,Rabbit |
Host Species |
Mouse |
Conjugate |
Unconjugated |
Applications |
Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form |
Purified |
Isotype |
IgM |
Gene Accession No. |
P63261 |
Concentration |
1.0 mg/mL |
Antigen |
F-Actin |
Gene Symbols |
ACTG1 |
Regulatory Status |
RUO |
Purification Method |
IgM purified |
Dilution |
Western Blot 1:100-1:500, Flow Cytometry 1:10, ELISA 1:10, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500 |
Gene Alias |
actin, cytoplasmic 2, DFNA26, ACT, ACTG, actin, gamma 1, BRWS2, DFNA20 |
Gene ID (Entrez) |
71 |
Immunogen |
Human monocytes and U937 cell line |
Classification |
Monoclonal |
Primary or Secondary |
Primary |
Test Specificity |
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types. |
Clone |
NH3 |