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Hot Start PCR Reagents and Kits

Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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1 – 13 of 13 results
1

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
GREENER_CHOICE

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Promotions are available
EDGE
Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
2

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Promotions are available
EDGE
Polymerase AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
3

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Promotions are available
EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
4

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
GREENER_CHOICE

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Promotions are available
Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
5

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.

Promotions are available
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Size (Final Product) 1.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
6

Applied Biosystems™ Platinum™ Multiplex PCR Master Mix

The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.

Promotions are available
Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR,Multiplex PCR
Multiplex Capability Up to 20-plex
7

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Promotions are available
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Product Type DNA Polymerase With Buffer I
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
8

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Promotions are available
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR
9

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

10

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2

Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Purity or Quality Grade LD (Low DNA)
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
11

Applied Biosystems™ True Allele™ PCR Premix

Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.

Promotions are available
12

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Promotions are available
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type Hot Start DNA Polymerase
Reaction Speed Fast or Standard
Overhang 3'-A
Concentration 2X
13

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
GREENER_CHOICE

Contains everything required for successful PCR amplification in a convenient package—all components are premixed and premeasured